That Night is the fifty-seventh episode of the anime.
Nina remembers when she and Johan were adopted by the Lieberts and moved from the East to West Germany and, how, one night, Johan killed the Fortners after saying that monster had came to take Nina and Johan away that night. Afterwards, Johan asked Nina to shoot him in the forehead.
Tenma arrives at The Red Rose Mansion, that has already been burnt. There, the police finds a grave with bones from the 46 people disappeared at the mansion. Tenma is then asked by two men to come with them to see General Wolf at the hospital.
A dying General Wolf tells Tenma that he gathered little information about an experiment going on at The Red Rose Mansion and that Johan was probably "born" there. Wolf also tells the story of how he found the twins at the Czechoslovak border with only a bag and the picture book Obluda as their possessions. Wolf gave Johan his name because of the book, which probably awoke something inside of him.
Nina, Dieter and Jaromír Lipský visit The Red Rose Mansion's ruins and then decide to create happy memories, so they go around Prague having fun. Back at Jaromír's place, he tells Nina that he has created a new marionette show about a girl who has forgotten her memories. Nina and Dieter depart.
- Nina Fortner
- Johan Liebert
- The Lieberts (flashback)
- Jaromír Lipský
- Rudy Gillen
- Julius Reichwein
- Helmut Wolf
- Heinrich Lunge
- Helmut Wolf: "Please say my name. That is the proof of my existence."